National Research Centre

Medical Research Ethics Committee

MREC Reviewer Comment

Protocol Title

Mitigation of Antimony-Induced Hepatic and Nephrotoxicity in Wistar Rats through Gelatin: Modulation of NFκB, TNF-α, and mTOR Gene Expressions

Completely satisfied and informative data

Needs Explanation

Comments to the applicant

- Protocol Title in English: Replace through by (using).

- Protocol Title in Arabic: Need to rearrangement of speech

التخفيف من السمية الكبدية والكلوية الناجمة عن الأنتيمون في فئران ويستار باستخدام الجيلاتين: تعديل التعبير الجينى لجينات NFkB ، TNF-α ، و          TOR

- Principal Investigator Data: Role/tasks in the research/project: Written 3 researchers other than the PI.

- Duration of the study: Written 2 months. While as mentioned in Procedure and Gant-Chart 10 weeks (2.5 months). And the proposal requires at least 3 months.

- Fund amount: Written Self , without writing an amount.

- Research Team: Co-Investigator: Written PI Name: Asmaa M. Fahim only. How ?, You written in Role/tasks in the research/project 3 Authors (Dr/ Naglaa A. Ali, Dr/ Ghada H. Elsayed, and Dr/ Mona Samir Hashem).

- Procedure in Details: Animal grouping and treatment design: Written in Group 4 Compound M3. You didn't explain what it is M3.

- Dose of M3 in Group 4, You did not write on what basis you chose this dose.

- Sample size and groups: Repeated more than once.

- Gant-Chart: written in 2 designs, can be satisfied with the first one.

- Flow-Chart: Repeated Gant-Chart.

- Does this protocol involve the use of substances that may pose any health risk: Written No. How ?, You will use Toxin element, blood, and tissues (liver and Kidney) samples.

- Dose your study involves collection of biological samples: Written tissue. did not write any tissues. And did not write blood.

- Agent/Substance: The speech is repeated more than once.

- What is the expected time course of the study: Written 2 months. How ? , as mentioned in Procedure and Gant-Chart 10 weeks (2.5 months). And the proposal requires at least 3 months.

- The title of the proposal differs from what is written in Form (How to fill the Application for Research Studies using Animals?)  than in the proposal online.  

(Amelioration of Antimony-induced hepatic and nephrotoxicity by Gelatin is mediated by lowering NFκB, TNF-α and mTOR gene alterations in Wistar rat.)

  تحسين السمية الكبدية والكلوية الناجمة عن الانتيمون بواسطة الجيلاتين عن طريق خفض التغيرات الجينية NFκB و TNF-α و mTOR فى الجرذان

Comment to the Committee

Informed consent form:

N/A

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