- Protocol Title in English: Correct peals to peel.

- Study Type: You wrote Cell line (human/ animal). The study did not contain cell line. Delete it.

- Keywords: Correct peals to peel.

- Sample size and groups: You wrote the daily use of mouthwashes for 2 minutes per day for 2 years. While, Duration of the study is 6 months ?

- Flow Chart file: You show the 3 steps take 13 weeks (3.25 months). While, You wrote Duration of the study is 6 months ?. Correct it.

- List of References: It very long and contain many references did not presence in the text. You must write the references that presence in the text only.

- The site and date of collection of plants: You wrote were obtained in June 2023. How, You wrote in Anticipated Start Date: The work will start after completion of all necessary approvals. Correct it after you obtain the approval or in June 2024, because season of these materials.

- Biosafety Issue: You did not mention any safety precautions, Although the study includes work with chemicals and microorganisms.