MREC Reviewer Comment
Protocol Title
Completely satisfied and informative data
Comments to the applicant
- Title in Arabic : استبدال كلمة حيوية بكلمة على الحيوان
- Anticipated Start Date: You wrote the work will start after completion of all necessary approvals. And wrote 2024-07-01. Correct date
- The protocol was reviewed by scientific reviewing committee: You wrote No.
- Keywords: Added mice.
- Procedure in Details: 3. In vivo study: 3.1. Median lethal dose (LD50): You wrote 186 adult albino mice weighing 20-25 g were divided into 31 groups, with 6 mice in each group. It is wrong whereas, 4 extract with 9 levels (4 X9) = 36 groups. With using 6 mice in each group (36 X6) = 216 mice.
- Grant-Chart File: Determination of the toxicity induced in mice by different doses of plant extracts. You draw it take Third - Fourth Week. While You wrote treated extended for 30 days. Added Fifth - Sixth Weeks (Third - Sixth).
- Does this protocol involve the use of substances that may pose any health risk: You wrote No. How, the work included tissue, fluids, blood, recombinant DNA, chemicals.
- Application for Research Studies Involving Animals: Total Number: You wrote 45. How, In vivo study: 3.1. Median lethal dose (LD50): You wrote 186 adult albino mice weighing 20-25 g were divided into 31 groups, with 6 mice in each group. It is wrong whereas, 4 extract with 9 levels (4 X9) = 36 groups. With using 6 mice in each group (36 X6) = 216 mice + 45 mice (9 groups x 5 mice) = 261 mice.
- There is a repetition in many places, what mentioned in Procedure in Details have been repeated in other sites (Sample size and groups, Justification of Animal Use, Describe all procedures on the animals and how often they will be done.
- What is the expected severity of pain the animal will suffer in your study?. You wrote No pain. How, Mice will be housed in nine cages, with five mice per cage. The mice will be orally treated using a stomach tube. And You will take blood samples. Also, in (Are less painful or stressful alternative available - Describe the anticipated pain or distress for animals - Describe how pain or distress will be monitored).
