Dr. Siham Soliman    Affiliation: NRC …. Needs more information

- Co-researcher Dr. Sharif Mohamed Afifi, and his email is: Naglaaammar@yahoo.com      Please revise the e-mail address

- The Author mentioned that the proposal was not reviewed by a specialized scientific committee, while it was revised technically by the department

- The Author mentioned that the ages of studied Buffalos were from 4-7 years and their weight ranged from 400-5500 kilos, and this is impossible.

- I think this research was completed because the work was from January to April, 2023 and this is against the regulations of the ethics committee from the beginning of this year.

- The Author mentioned in the experiment procedures that the ages of the studied Buffalos were from 4-7 years, and in another place she mentioned that the ages were 3-5 years.

- The Author did not mention the size of the blood sample, but mentioned that she will take them in a 1.5 mm Eppendorf tube? In another place, she mentioned that she will take 10 ml of blood sample/animal.

- She stated that there was no pain even though she took blood from the jugular vein.