1- Arabic title sounds wrong

2- The investigators Ahmed  abdelfattah and Samy Ibrahim, their roles (blood sampling, injection, sacrifice, analysis)do not match their expertise (writing).

3- The groups need justification for inclusion of each of the 4 control groups (20 rats).

4- The background is very deficient, for example, there is no mention of side effects, carcinogenecity, benefits of vesicle over nonvesicles etc. Moreover, the background mentions that nonvesicles are better than vesicles used for the current study.

5- Who will prepare the creams?

6- What is the justification for using males and wistar strain. Wistar is written is written wister in some places.

7- The aim mentions inflammation due to UV, while title is photoaging

8-Pain, don't UV inflammations cause pain?

9- Pain, during decapitaion, how to reduce it should be mentioned here.

10-Monitor distress is mentioned "by edcapitation", while in pain section decapitation is mentioned as a cause of pain (and distress).

11- "Drag" analgesic, should be drug. This part should be also mentioned in the sacrifice and decapitation section.