MREC Reviewer Comment
Protocol Title
Purslane Leaf Extract: A Novel Therapeutic Approach to Mitigate the Effect of 4 (Methylnitrosamino)-1-(3-pyridyl)-1-buta-4 None (NNK)-Induced on Testicular Histopathological Alterations and Hormonal Imbalances in Male Rats.
Completely satisfied and informative data
Needs Explanation
Comments to the applicant
Flowchart was not attached
Authors have approval from the department and institute, but they say their research work was not reviewed.
The authors need to assess the cytotoxicity of Purslane extract and NNK doses. They should perform either an LD50 test or an in vitro assay, such as the MTT assay, to determine the half maximal inhibitory concentration (IC50).
The authors did not address the risks of their work.
Comment to the Committee
Informed consent form:
N/A
