The protocol was reviewed by scientific reviewing committee: You wrote No.
Keywords: Added mice.
Mention the source of cell lines
Procedure in Details: 3. In vivo study: 3.1. Median lethal dose (LD50): You wrote 186 adult albino mice weighing 20-25 g were divided into 31 groups, with 6 mice in each group. It is wrong whereas, 4 extract with 9 levels (4 X9) = 36 groups. With using 6 mice in each group (36 X6) = 216 mice.
Does this protocol involve the use of substances that may pose any health risk: You wrote No. How, the work included tissue, fluids, blood, recombinant DNA, chemicals.
Application for Research Studies Involving Animals: Total Number: You wrote 45. How, In vivo study: 3.1. Median lethal dose (LD50): You wrote 186 adult albino mice weighing 20-25 g were divided into 31 groups, with 6 mice in each group. It is wrong whereas, 4 extract with 9 levels (4 X9) = 36 groups. With using 6 mice in each group (36 X6) = 216 mice + 45 mice (9 groups x 5 mice) = 261 mice.
What is the expected severity of pain the animal will suffer in your study?. You wrote No pain. The mice will be orally treated using a stomach tube.