National Research Centre

Medical Research Ethics Committee

MREC Reviewer Comment

Protocol Title

Protective Effects of Dietary Purslane (Portulaca oleracea) Extract on Growth Performance and Biochemical and immunological Responses of Nile Tilapia (Oreochromis niloticus) Exposed to Lead Toxicity

Completely satisfied and informative data

Needs Explanation

Comments to the applicant

Did not mention the fund amount

Did not write the department and institute name.

Hypothesis, Rationale and Scientific Validity : Dietary supplementation with purslane extract will mitigate the toxic effects of lead exposure in Nile tilapia by reducing tissue lead accumulation, improving growth performance and physiological health, enhancing antioxidant defense mechanisms, and alleviating histopathological damage in a dose-dependent manner. (NEED reference). No ref.

  Fish will be anesthetized using an approved fish anesthetic before sample collection, please mention reference and methods including recommended dose. (did not ref.)

Comment to the Committee

Did not mention the fund amount

Did not write the department and institute name.

Hypothesis, Rationale and Scientific Validity : Dietary supplementation with purslane extract will mitigate the toxic effects of lead exposure in Nile tilapia by reducing tissue lead accumulation, improving growth performance and physiological health, enhancing antioxidant defense mechanisms, and alleviating histopathological damage in a dose-dependent manner. (NEED reference). No ref.

  Fish will be anesthetized using an approved fish anesthetic before sample collection, please mention reference and methods including recommended dose. (did not ref.)

Informed consent form:

N/A

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