National Research Centre

Medical Research Ethics Committee

MREC Reviewer Comment

Protocol Title

Phyto- and Biochemical Study on Garden Cress (Lepidium sativum Linn.) Seeds Extracts: in Vitro and in Vivo Studies

Completely satisfied and informative data

Needs Explanation

Comments to the applicant

He mentioned the protocol was not reviewed by scientific reviewing committee although he attached the form 90

The co-author not in the field of research just to write the paper

He did not mention from where the  human hepatocellular (HEPG-2), colon carcinoma (CACO-2) and lung cancer (A549) cells 

In methodology No. of mice in measuring LD50 186 plus 45 for the experiment

He mentioned in Animal Housing Requirements Two hundred sixteen (216) adult albino mice weighing 20-25 g were divided for calculating the LD50

He mention severity of pain no pain although the mice will be treated by stomach tube and the blood samples from  Retro-orbital plexus

Comment to the Committee

He mentioned the protocol was not reviewed by scientific reviewing committee although he attached the form 90

The co-author not in the field of research just to write the paper

He did not mention from where the  human hepatocellular (HEPG-2), colon carcinoma (CACO-2) and lung cancer (A549) cells 

In methodology No. of mice in measuring LD50 186 plus 45 for the experiment

He mentioned in Animal Housing Requirements Two hundred sixteen (216) adult albino mice weighing 20-25 g were divided for calculating the LD50

He mention severity of pain no pain although the mice will be treated by stomach tube and the blood samples from  Retro-orbital plexus

Informed consent form:

N/A

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