1)Modification of the title . 2)Please complete the affiliation of Teamwork(Department and Institute ).3)Mention the precautions that will be followed when using Streptozotocin to minimize health risk. 4)The tested doses of oil and different extracts need confirmation by references or toxicological study. 5)Flowcharts are not described. 6)The name and origin of analgesic and anesthetic drugs used in the experiment are not mentioned. 7) collection of 15 ml of blood sample from rat weighing ( 150  gm) is an exaggerated volume.8)Biochemical tests are not mentioned.