1)Please specify the area of expertise of the PI. 2)The tested dose of the new synthesised drug( 5mg/kg(b.wt,) ) must be determined after previous toxicological study for safety.this test is not described in the article. 3)Please complete the precautions that will be followed to minimize health risk for humans and animals when using the toxic agent STZ. 4)More details are required in the Flowchart .