Report

Protocol Title in English:

Effect of eugenol cationic guar β cyclodextrin poly electrolyte complex nanoparticles in pentylenetetrazol-induced epilepsy in mice

Protocol Title in Arabic :

تأثير الأوجينول–الغوار الكاتيوني–بيتا سيكلودكسترين متعدد الإلكتروليت على نموذج الصرع المُستحث بالبنتيلين تترازول في الفئران

1-    تعديل ترجمه العنوان العربى الى:

تأثير جسيمات النانو المعقدة لجوار الكاتيوني يوجينول β السيكلودكسترين بولي الإلكتروليتات في الصرع المُستحث  بالنتيلينيترازول في الفئران.

2-The Gantt Chart indicated the experiment duration was one month, not 15 days, scheduled from April 1 to April 30, 2025, so this work was completed last year.

3-The actual budget was not provided.

4-The team's role is unclear.

5-There is no designated person for nanoparticle preparation, and it is not mentioned whether they will purchase from a company or where they will source it.

6-The procedure for developing polysaccharide-based nanoparticles is absent and lacks any references.

7-What are the references for the doses of pentylenetetrazol, eugenol, and the polysaccharide-based nanoparticles used in the experiments?

8-The study should also include a toxicity analysis, specifically focusing on the LD50  and LC50 of the drugs.

9-The sample size calculation needs to be referenced differently than Fraz Faul et al. (2007) since it pertains to statistical analysis, not these experiments