Report

Title in English:

Design and evaluation of an intranasal nanoformulation for conjugated linoleic acid for the treatment of pentylenetetrazole (PTZ) induced seizures in miceProtocol

Title in Arabic :

تصميم وتقييم صياغه نانومتريه عن طريق الأنف لحمض اللينوليك المترافق لعلاج نوبات الصرع المُستحثة بالبنتيلين تترازول في الفئران

Primary Objective (s)

Developing an effective nano-formulations for conjugated linoleic acid (CLA) encapsulated into polymeric formulation to be employed intranasally for neuroprotection and to mitigate PTZ induced epileptic seizures

Secondary Objectives

Design an effective, safe, and convenient therapy for epilepsy Investigation of neuroprotective effect mechanisms in PTZ induced epilepsy in an animal model

Comments:

1. The English title will be changed to:

Design and evaluation of an intranasal nanoformulation for conjugated linoleic acid for the treatment of epileptic seizures in mice induced by pentylenetetrazole (PTZ)

2. The duration of work requires more than one week; one week is needed for treatments, and additional time is necessary for biochemical and histological studies.

3. The funding amount was not specified.

4. The methods for preparing nano-formulations of conjugated linoleic acid (CLA) encapsulated in a polymeric formulation need to be referenced or the source must be mentioned, or the preparation methods should be explained.

5. Procedure details:

a. References are needed for the system used to induce epilepsy in mice and for the doses of pentylenetetrazole (PTZ).

 b. Toxicity studies, including LD50 and LC50, must be conducted for both the PTZ and the nano-formulation before use, or appropriate references must be provided to ensure their safety for mice.

 c. A total of 48 animals will be used, divided into 6 groups. What reference standard will be used for the 3rd group, and which blank formula will be used for the 4th group?

d. The source or preparation method of the nano-formulation and its characterization were not mentioned.

e. Clarification is needed on how the nasal spray will be prepared and how the dosage for the animals will be calculated.

f. The Gantt and Flowchart designs are incorrect.

g. The assessment of epileptic seizures requires a reference.

h. References must be provided for the biochemical analysis of MDA, GSH, and GABA from brain tissue, and the abbreviations of these chemicals should be defined.