1. The authors indicated that they would begin their work after receiving acceptance from the Ethics committee; however, the work is already completed. They have documented the results of the study.

 2. The date on the signed 90 form is incorrect; it shows 9/10/2025.

3. There is a lack of clarity regarding the number of animals used. At one point, the authors mention 250 mice, while at another, they state 90 mice divided into 9 groups, with each group containing 10 mice. In another instance, they mention 45 mice divided into 10 groups, with each group having 5 mice.

4. The budget amount is missing.

5. The authors need to specify the frequency and duration of the injections with Co-Ni-B NPs and Fe₃O₄ NPs.

 6. The method proposed for disposing of deceased animals is inadequate, as the authors plan to bury the mice. Further details on how and where this will take place are needed.