National Research Centre

Medical Research Ethics Committee

MREC Reviewer Comment

Protocol Title

Flax seed protein fractions regulate the proteasomal degradation pathway through suppression of FOXO signaling and oxidative stress, thereby preserving muscle integrity

Completely satisfied and informative data

Needs Explanation

Comments to the applicant

-Add fund amount.

The first main group (healthy animals)

-Subgroup 3: It is mentioned that ten rats will be administered peanut protein (hydrolyzed fraction), what is its source?

The second main group (diseased animals) 

- It is mentioned that the second main group will be diseased animals (40 rats) that were orally administered Corvast, add that this is simvastatin market product, and add company name.

- It is mentioned that Subgroup 2: ten rats will be administered isolated protein (1 g/kg/day for 15 days orally) and will be kept in a peanut isolated protein-treated group. Subgroup 3: ten rats will be administered the hydrolyzed protein (1 g/kg/day for 15 days orally) and will be kept in a peanut hydrolyzed-treated group, clarify what will be administered by these subgroups.

-The group administering Flax isolated protein is not mentioned.

* What are the parameters which will be measured in blood samples?

* In the Gantt chart, it is mentioned that treatment by force-feeding the protein daily at a dose of 1000 mg/kg, clarify how force-feeding will be done, and add what will be administered to induce muscle atrophy.

And it is mentioned that treatment by force-feeding the extract daily at a dose of 500 mg/kg, which extract? also this dose is not mentioned anywhere in the proposal

*In the flow chart, it is mentioned that Induction of muscular atrophy by administration 80 mg/kg body weight orally for tow weekly, clarify what will be administered and correct spelling mistakes.

- it is mentioned that:  We performed a pilot study to discover the statin-muscular atrophy model because this is the first experience in this model, while this model is presented in literature.

- In animal disposition item, add the answer
- The protocol is full of spelling mistakes, please correct.
 
نموذج 90 غير موضح به إذا كان البروتولول سليم علميا و تمت الموافقة عليه أم لا كما أن إصدار النموذج قديم
 

 

Comment to the Committee

Informed consent form:

N/A

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