The protocol needs modifications:

1-Title: The English title is incomplete (the word "fertified" must be followed by "by" or "with" to clarify the additions to this burger).

2- The scientific background is short, lacks references, and does not discuss the ingredients added to the burger under study.

3-The ingredients under study that will be added to the homemade burger were not specified.

4- In the experimental procedures section, the methods (biochemical and histology) that will be used to complete the practical part were not explained.

5. The Gantt chart and flow chart were not created correctly according to standard procedures.

6- Sample size calculation: The reference used in this calculation was not provided.