The protocol needs modifications:

1- The study period is one week!!!!

2- The funding was not specified (it says "self-funding").
3- Researcher number 2 in the protocol is named Mona Mohamed Agwa Affiliation.
4- In the experimental design section, it is unclear how group 4 will be treated (the study will consist of 6 groups).
5- The sample size and groups section was duplicated in the experimental design section.

6- In the section "How to explain how you calculated the sample size," a reference to the same group on Nanospri was included.
7- The Gantt chart uploaded was incorrect; it is more likely a flow chart.
8- In the section "Mice Breed," it says "Swiss" instead of "Swiss Albino mice."
9- In the section "Justification of Animal Number," it says 5 groups of mice, not 6 as stated in the rest of the protocol. Many sentences are also repeated multiple times.
10- In the section "Describe all procedures," it states that there are 40 mice, which is different from the rest of the protocol. Many sentences are also repeated multiple times.
11- The analyses to be performed on the tissues are not explained in detail but are listed as headings.
12- When asked about the pain level, the answer was "minimum," even though the mice will experience seizures.