The protocol needs revisions:

1- From a scientific standpoint: The study idea is very old and offers no new scientific contribution – even the techniques used are outdated.

2- Department and institute information is missing for the principal investigator.
3- It states that the protocol has not been reviewed, despite the existence of Form 90.
4- Funding is not specified (it says "self-funding").
5- The techniques used in the study are not explained; only headings are provided.
6- The Gantt chart indicates a study duration of 6 months, but the protocol states 3 months.
7- The flowchart is incorrectly designed (in slide format).
8- In the references section: A very large number of references are listed, but only 4 are included in the protocol text.
9- Explanation is needed regarding the selection of female rats for the study.
10- The animal handling form has not been completed and uploaded.