The protocol needs modifications:

1- The English title : Instead of: ERK1/2/Nrf2, NF-κB/TNF-κB, and Angiotensin-II/TGF-β1 Signaling Pathways,” should write “Three signaling pathways for cellular stress, inflammation, and tissue remodeling.”

العنوان العربى: ...  تنظيم ثلاثة مسارات تتحكم فى الإجهاد الخلوي والالتهاب وإعادة تشكيل الأنسجة

كتابة جرذان بدلا من فئران

2- Department and institute data are not included for the principal investigator.

3- Research participants: 2 (Dr. Lamia Ramadan from the center and Dr. Dina from Mansoura University Faculty of Medicine).
4- When asked about funding, the answer was "self-funding" without specifying the research budget.
5- The primary and secondary research objectives were not clarified.
6- The background section is very short and contains no scientific references.
7- The experimental procedures are very brief and do not mention the analyses to be performed.
8- The calculation of sample size was not explained.

9- The references section includes six references that are not found in the protocol text.