In which department is the PI working, and what is the background of the PI (PhD in what?)

Study type: is mentioned “observational” “cohort” This is not correct.

What is the funding amount?

Investigators Farah Hany Ashraf and Nada Wael Youssef, affiliation is STEM

high school and the qualifications are not impressive to be able to do data collection.

The groups need to be numbered or have a letter designation for clarity.

How come the positive control does not receive any treatment, while the negative control receives gel base only. Usually, negative controls receive no treatment.

In the “pain” section blood sampling is mentioned, while in the methodology blood sampling is not mentioned.

The inflicted would is a source of pain. How will this pain be managed?

The methodology is very deficient and does not explain how the wound will be inflicted, how big, how deep, who will perform them, how will the samples be collected, what will happen to the rats at the time of tissue sample collection, will they be euthanized before tissue sample collection or not etc.