- Principal Investigator Data: Institute and Department: Written numbers.

- Position: Written other, without writing the job name.

- Duration of the study: Written 3 months. How, this proposal is a Ph.D. Thesis.  And in the Gantt-chart, Written the study will take One year (12 moths).

- Funding Agent: Written self. How It is a Ph. D. Thesis. And it is funded by NRC (Thesis fund).

- Fund amount: Written number 3 only without specifying excellence (pound, thousand, million).

- Procedure in Details: Written A one hundred Male Wistar adult rats (10 rats per group). While, written in Sample size and groups 6 groups (60 Rats) only.

- Groups: Written Group III and IV together, Group V and VI together. This made confusion. They must be separated as G III, G IV, G V, G VI).

- The part of the plant used is not written.

- It is not written how to prepare plant extracts.

- Dose: Dose of 50% ethanol solution (10 ml/kg), and Chiliadenus Montana methylene chloride to methanol, 70 % methanol, Chiliadenus candicans methylene chloride to methanol (400 mg/kg). It is not written on what basis were used.

- Not written in procedure it will be conducted Histopathology, Biochemical analysis ( MDA, GSH, NO ICAM, IL 18, NRF2, HO 1, VEGF, MPO), Sacrifice and Tissue preparation for rats.

- In question of (Does this protocol involve the use of substances that may pose any health risk): written No. How, in this study, we will use chemicals (solvents, …), blood, tissues

- Effective dose: written 200 or 400 mg/kg. While the higher dose was used (400).

- The site and date of collection of plants: written both plants were collected in May 2018. So far in 2025, it has been seven years since the plants were collected. How does this affect the effectiveness of the active compounds found in the plants?

- Animal handling form does not exist.