- Protocol Title in Arabic: اضافة صديقة للبيئة اخر العنوان ، ليتوافق مع العنوان الانجليزى

- The name of the Institute and the Department is written in numbers. Correct it.

- Fund amount: Written National Without specifying an amount of money.

- Research Team: Co-Investigator number 7 and 8 are Foreigners, Security clearance required.

- Co-I. Number 7: Written the French co-ordinator and introducing the extracted pigments in industrial Trials. How and it is written in Co-I. Number 2 and 5 (Egyptian Team), He will isolation of micro-organisms and production of the pigments. Which one is correct?

- Co-I. Number 8: Written A French co-ordinator and performing the industrial applications of the pigmented extracts as food colorants. The question is whether this part of the work will be carried out only in France.

- Co-I. Number 9: Written PI (Name and Data). This is not his place.

- Reference list: Is divided into several parts. It should all be combined into one list.

- Procedure in Details: Skin irritation test and in Sample size and groups: Written using Wistar female rats. First, the question is why use females. It is better to use males. Second, the number of animals used was not mentioned.

- In answer to a question (Does this protocol involve the use of substances that may pose any health risk). The answer No. How, the work involves using animals, Chemicals, Fungi, Blood samples, Urine samples, and Internal organs.

- Application for Research Studies Involving Animals: Total Number: Written 150 rats. First, this number is very large. Second, it is different from what was mentioned in the previous sections.

- In answer to a question (Dose your study involve collection of biological samples): The answer Blood, organs. First, did not mentioned what the organs are. Second, did not mentioned taking urine samples, as previously mentioned. Thirdly, did not mentioned the parameters which will determined. Fourth, did not mentioned performing a histological examination of the organs, which is very necessary.

- In answer to a question (Are less painful or stressful alternative available). The answer No.

- In answer to (Describe the anticipated pain or distress for animals). The answer No detectable pain. How?

- In Experimental Procedures: Written all biochemical markers will be performed. Do not mentioned the parameters which will determined, in blood, urine, and in organs.

- The PI written in detail about use of produced pigments as a textile Colorant. However, He did not write about use it as a Food Colorant.

- Attachments: Form 90:

مكتوب فى النموذج معهد: بحوث الصناعات الكيميائية. قسم: كيمياء المواد الدابغة وتكنولوجايا الجلود.

-نموذج اقرار الباحثين بالرعاية الجيدة للحيوانات

مكتوب فى نوع الحيوان المستخدم والعدد: عدد 150 جرذ. اولا: هذا العدد كبير جدا. ثانيا: هذا العدد مختلف كثيرا عن ما جاء فى تفاصيل العمل (اجمالى عدد 50 ). رجاء التاكد.