-The name of the institute and the department is not written.

- Submission Type: Written Re-submission.

- Fund amount: Written Local Without writing an amount.

- Research Team: The proposed project is funded by Academy of Scientific Research and Technology " within the agreement on scientific cooperation between ASRT Egypt and the Hungarian Academy of Sciences”. Despite that, there are no members from Hungary.

- There is no specialist in animals in the research team.

- Procedure in Details: Animals: Written thirty virgin female rats. And One adult male rat will then be introduced into each cage (1 male:3 females). This is contrary to what was mentioned in Experimental protocol (forty and 1 male:4 females).

- In Procedure, Pregnant females: Delete (4 ± 1 males and females) by random selection (Fischbeck and Rasmussen, 1987).

- In Procedure: Written the body temperature of rats from each group will be is measured every hour for 24 h. This is too much and causes stress and pain.

- In Procedure: There is no indication of the pesticide estimate either in the milk or in the newborns. This is very necessary.

- Before Sample collection: paragraph (All doses will be prepared ……. maternal milk). Duplicate, must be deleted.

- In Serum biomarkers: Written Liver function will be assessed. The kidneys function is not written, and this is very important.

- Gantt Chart: Incomplete writing (Part of the writing is not visible).

- References: There is a big mess in it, There are 21 redundant references that must be deleted.

- Statistical analysis: Written Data will be analyzed using One-way analysis of variance. This is not correct. whereas in this study two variables are studied, not one variable. Based on that, Two-way is the correct. And this is written in Justification of Animal Use.

- In Describe the nature of the experimental agents used in your study: Written No. How?

- In answer to a question Dose your study involves collection of biological samples. Written No. How, written in other places he will take samples (liver, kidneys, brain, and spleen).

- In Describe the anticipated pain or distress for animals: Written None. How?

- In Describe how pain or distress will be monitored: Written None.

- In List who will monitor or observe animals: Written None. 

- In Indicate the schedule of monitoring: Written None. 

- In Animal Housing Requirements: The required was not written, but write Please refer to the protocol.

- In Animal disposition: The required was not written, but write Please refer to the protocol.

- In Identify and explain if any individual animal in this project will be used in any other project: Written Please refer to the protocol.

- In What will be the method of disposal of dead animals: The required was not written, but write Please refer to the protocol.

- In Will researchers perform technical procedures on animals in addition to routine husbandry: Written No. How?