MREC Reviewer Comment
Protocol Title
Effect of Fenofibrate on thioacetamide-induced hepatic encephalopathy in rats
Completely satisfied and informative data
Needs Explanation
Comments to the applicant
NO
Comment to the Committee
Regarding Sample Size Justification:
The protocol states 32 rats (8/group) but cites a calculation requiring 12/group (90% power, effect size 3.1). Later, it mentions 80% power without clarification. Please clarify the rationale for using 8/group instead of 12 and power (80% vs. 90%) and Provide updated justification.
Regarding Euthanasia:
Midazolam Use: Midazolam (15 mg/kg ) is a sedative, not an anesthetic. Cervical dislocation under sedation alone may not comply with the 3 Rs guidelines.
Informed consent form:
N/A
