MREC Reviewer Comment
Protocol Title
A Novel Nanotherapeutic Protective Approach Using Chitosan-Coated Biosynthesized Selenium Nanoparticles Against Gentamicin-Induced Hepatorenal Damage in a Rat Model
Completely satisfied and informative data
Needs Explanation
Comments to the applicant
NA
Comment to the Committee
1- The use of Diethyl Ether poses major safety and ethical concerns. The protocol also includes Ketamine at 10 mg/kg for euthanasia/anesthesia ????
2- In the Study Duration and Timeline: The protocol states in the "Procedure" section that the experimental period is "planned for 8 days." However, the Group descriptions state:Control: 24 days.
3- Blood Collection Site: Collection via "puncturing sub-lingual vein."Comment: This method can be technically difficult and stressful if not done by an expert.
Informed consent form:
N/A
