1- Affiliations, experience and roles for Co-Is are missing
2-Objectives are not well formulated
3- There is no Null hypothesis, although the sample size calculation is written to reject the null hypothesis
4- Source mentions mature-male, while the methodology mentions females and males. Please correct the discrepancy.
5- The Procedures section does not mention what will be done after the treatment.
6- Animal disposition answer is euthenesia and not disposition.
Comments about the website
1-Once the comments view is open it cannot be closed to view all the protocol.
2-The comments cannot be saved for a later session
As for the protocol, it looks like a manuscript. Not sure if it was already performed or not, I am not sure.