·         I suggest adding The role of pectin improvement  and then .complete the title as it is

·         Study type not observational

·         Fund amount is determined approximately

·         The secondary objective is written as the same of the primary objective

·         When were t he blood samples taken from the mice after scarified them or before ?.Were the mice anesthetized before taking the blood samples? .Please review the  writing of this section  . (Finally, rats were fasted overnight and were anesthetized by injection ketamine and xylazine (87 and 13 mg/ kg of body weight) dissolved in normal saline. Anesthetic rats were scarified and the blood samples were collected from the retro-orbital plexus for biochemical analysis)

·         Explain the Sample size,  is it calculated or by reference?

·         Jantt chart  as the same flowchart

·         In the blood sampling: centrifuged at 3000 rpm for 15 minutes to obtain sera,  while in Describe all procedures on the animals and how often they will be done ;  Blood was centrifuged (4000 rpm for10 min . which is correct ?

·         Are less painful or stressful alternative available

·         Frequency: No  ( write mild pain duo to the pain of the syringe during injection to prepare the model, treatment administration orally).

·         Agent / Substance: Drug Tranquilizers: ketamine/xylazine Route of Administration Tranquilizers: oral ,.but in Animal grouping and treatment design: Anesthesia rats achieved by injection ofketamine 87 mg/kg of body weight and xylazine 13 mg/ kg of body weight, dissolved in normal saline, and each rat received 0.2 mL/100 g body weight