1-     The study duration is two weeks, which is insufficient for all analyses. The authors require two weeks solely for in vivo work.

2-     The funding for this project is currently unavailable, and the authors will self-fund the necessary expenses. They did not initially include the funding request in their proposal. The work involves the purchase of animals and plants, preparation of nanoparticles, surgery, biochemical analysis kits, and microRNA analysis. These items are costly, and the funding situation must be clarified.

3-     What is the source of Staphylococcus aureus? What reference count will be used for the infection, and which method will be employed to determine it?

4-     The authors should detail the preparation of Lycium shawi blank extract and the nanoparticle gel preparation or include references with a brief method.

5-     The authors will take tissue samples from the wound on the 2nd, 5th, 7th, 10th, and 14th days during the healing process. However, this frequent sampling may delay the healing. Therefore, I prefer that the tissue samples be collected only at the end of the experiment.

6-     The author did not explain the methods of glutathione and lipid peroxidase analysis, and the references mentioned are not included in the reference list. Moreover, the microRNA analysis is also missing.

7-     The authors believe there is no risk to humans, despite the risk of infection from S. aureus.