1-Idea is good and important. There are published research works similar to this topic and design.

2-Authors experiences can do the research work

3-The fund doesn’t mention.

4-How they will count the larvae and nymph that will be on the ear bags, what are  the references to choose 2000 larvae and 1000 nymph ? What is the duration of the infestation?

5-Chimeric antigen Q38-95 source is Spain is it purchased from company or it is prepared in the Spain lab (Brief explanation)?

6-The antigen combination efficiency will be used in cattle tick infestation, How they will do that?

7-The sequence for SUB and BM95 need references

8-Fig 1 is the flowchart

9-The blood will be collected to have the serum.(the authors wrote collection of the sera)?

10-The number of animals are 14 not 12 because they will use two rabbits for infestation the 12 rabbits for experiments.

11-There is pain due to the tick infestation.

12-In the end of the experiment the authors must treat the control animals using drugs for external parasites infestation.