Report

Protocol Title in English:

Formulation of artemisia annua loaded chitosan nanoparticles for oral use to treat cryptosporidium infection

Protocol Title in Arabic :

صياغة حبيبات الكايتوزان المحملة بمستخلص الشيح للعلاج عن طريق الفم لعلاج عدوى الكريبتوسبورديام

Objectives:

-Primary Objective (s)

Explaining the protective effects of Artemisia annua chitosan nanoparticles against Cryptosporidium infection

-Secondary Objectives

Discovering the possible underlying mechanism that mediates such an effect.

Comments:

1.      The English and Arabic titles must exclude the route of administration and include the animal species.

 Suggested title: -

Formulation of Artemisia annua-loaded chitosan nanoparticles to treat cryptosporidium infestation in mice.

- صياغة جسيمات الكيتوزان المحملة بالشيح لعلاج عدوى الكريبتوسبوريديوم في الفئران.

 2. The authors have agreement form No. 90; however, they stated that the proposal was not reviewed.

 3. The funding amount has not been specified.

4. The background and references provided are insufficient.

 5. References are needed for the methods of infestation and treatment.

 6. Clarification is required regarding the source of Artemisia annua chitosan nanoparticles. If they come from a company, the company name should be provided. If they will be prepared in-house, a brief methodology and reference should be included.

 7. The dosages of Nitazoxanide and Artemisia annua chitosan nanoparticles need references or toxicity test information before use.

8. The authors indicate that there is no pain, yet mention abdominal pain due to Clostridium infestation.

9. The dosage and type of anaesthesia drug to be used must be clarified.

 10. The Gantt chart is not clear and requires improvement.