1-The author submitted the necessary department and institute approvals, but mentioned that the research work did not undergo a review process.

 2. There is no funding amount provided.

3. The number of mice per group is currently set at three, which is insufficient for statistical analysis and accuracy of the results. It is recommended to increase this number to five or ten mice per group.

4. The authors plan to sacrifice the animals, so there is no need for a surgical operation.

5. The sterilization of the instruments used for tissue samples must be done in a hot air oven, not in an autoclave.