National Research Centre

Medical Research Ethics Committee

MREC Reviewer Comment

Protocol Title

Antimicrobial, phytochemical and cytotoxic properties of Okra leaves and Cactus fruit peals prepared mouthwash and related effects on the surface hardness of some dental resin composites

Completely satisfied and informative data

Needs Explanation

Comments to the applicant

Please give more details on the types of dental resins used.

In the methodology section, it is mentioned that "antimicrobial and cytotoxic effects of two edible plant waste mixtures are to be evaluated,"  yet there is no reference to this in the methodology section. How do you plan to assess these parameters? As for the cytotoxicity, are there cell lines involved?

How will the MIC values be determined? What are the specimens that will be tested?

In the " Sample size and groups" section. Please provide more details on the types of specimen in relation to each test and how the sample size was claculated.

The consent form needs to be reconsidered after clarification of the studied samples for cytotoxicity and antimicrobial evaluation.

Comment to the Committee

Informed consent form:

Needs Modification

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