-          Title  to be revised (remove “in”)

The protocol was reviewed by the scientific reviewing committee [No] : what about نموذج 90

-          Primary Objective (s) [Investigation of bioactive constituents from the plant for possible medicinal use via]

Comment: not clear

-          Secondary Objectives [To discover a new anti-cancer drug from a natural source]

Comment: not clear

-          Procedure in Details [Lung cancer induction: 40 adult male Sprague-Dawley rats weighing 180- 200 g will be underwent for lung cancer induction with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Rats in this group will be treated with a dose of 1.5 mg/kg body weight NNK subcutaneously three times weekly for 4 weeks]

Comment : induction of cancer is not a treatment

-          Gantt Chart: Portulaca-oleracea-Alcoholic-extract-موذج-التقدم-بأبحاث-تستخدم-الحيوانات-2-Copy.pdf

              Flowcharts: -المتعامل-مع-حيوانات-التجارب-.pdf

Comment: Gantt chart and flow charts are not uploaded

-          Does this protocol involve the use of substances that may pose any health risk (infectious, carcinogenic, toxic, bacteria, viruses, fungi, parasites, cell lines, primary cells, tissue, fluids, blood, recombinant DNA, chemicals, laser or radiation) to humans? No

Comment: The protocol includes use of chemical substances, one of them is carcinogenic. The research team should be aware of the possible hazards and protective precautions.

-          Sample size calculation [Reference]

comment: citation of reference and description of animal groups are required.

-          What is the expected severity of pain the animal will suffer in your study? Frequency: No pain

Comment: Cancer research had severe pain. Please check the degrees of severity of pain  

-          Describe the anticipated pain or distress for animals [Frequency: The pain of the syringe during injection to prepare the model, treatment administration orally]

Comment: the pain of the cancer disease should be considered

-          Animal disposition

Comment: bags should be labeled and frozen before sending them to OSH committee