- English and Arabic titles should include “Wister-albino rats”

"The blood samples were collected from the eyes every 3 days and blood glucose levels will be determined by using the suitable blood glucose detection kit."
- How many times will the blood specimens collected from the eyes of living animals during the period of the study (one month). Is there a harm to the eyes from this frequent blood sampling?

Reference: valuation of anti-diabetic potential of chromium(III) propionate complex in high-fat diet fed and STZ injected rats

- What is the reference of sample size?

The flow chart is not clear and does not include the four rat groups, drugs, doses, durations, specimens, ... for each group.