1. Funding Agent is Self and Fund amount is Self: The expected fund amount should be stated as the study includes costs of the PCR and identification of bacteria.
2. Will you include equal numbers of males and females?
3. How many fertile eggs will be included? Please emphasize, noticing that 60 chicks will be included.
4. The flow chart is not clear. It should emphasize the number/groups of chickens, fertile eggs, males, and females to be included in the study.
5. What will be the method of disposal of dead animals? Burning: Where will you burn the dead animals?