The procedure he /she is responsible for:

He uses the verb “do” excessively. For instance, instead of saying “She will do the collection and authentication of plant materials,” he should rephrase it to “She will collect and authenticate plant materials.”

Procedure in Details
Further information is needed on the following aspects:

1. Plant part utilized

2. Source location of plant collection

3. Extraction methodology

4. Experimental design

5. Tissue sampling procedure

Sample size and groups
 

The study involves 30 rats divided into 5 groups of 6 rats each. The group allocation is as follows:

1. Group 1: Negative control group (healthy rats without gastritis)

2. Groups 2-5: Rats with ethanol-induced gastritis

    - Group 2: Positive control group (no treatment)

    - Group 3: Reference drug group (treated with a standard medication)

    - Group 4: Treatment group receiving extract at a dose of 100 mg/kg

    - Group 5: Treatment group receiving extract at a dose of 200 mg/kg

However, the study lacks clarity on two crucial aspects:

- The methodology used to induce gastritis in rats using ethanol

- The specific extract being administered to the treatment groups (Groups 4 and 5), with only the doses (100 mg/kg and 200 mg/kg) being mentioned.

Gantt and Flowcharts
The participant mentioned that the duration of the study will be 3 months in the headline “the duration of the study”, 13 months in the title of Gantt chart and 10 months inside the Gantt chart table. Revise and write the correct time in all situations.
 
Describe all procedures on the animals and how often they will be done
 The text was written in the past tense.