The protocol needs modifications:

1- The title:

كلمة استخدام ليست ترجمة دقيقة لكلمة

"employment" 

 (توظيف)

2- The scientific background is short and lacks references. It also does not discuss the topic of the thesis.
3- It does not explain why female rats were used and not males.
4- In the group division, there is no difference between Group 1 and Group 2 (same treatment).
5- In the experimental procedures, the number of groups is 3, but the flowchart lists 6 groups.
6- In the sample size collection section, only the reference is mentioned (and it does not specify which one).
7- It is clear that the type of nanoparticles used is either selenium or chitosan (it must be specified because it will determine the number of treated groups of rats).