The protocol needs modifications:

العنوان : العنوان العربى ينقصه كلمة "صديقة للبيئة" (إنتاج أصباغ ميكروبية مستدامة وتطبيقاتها المحتملة كملونات صديقة للبيئة للمنسوجات والأغذية)  

2- Two members of the research team are from France.

3- The principal investigator's data is written as numbers (department and institute).

4- The research team does not include a specialist in textile research, even though a large part of the project focuses on textiles.

5- The practical part of the experimental procedures focused only on fungi, even though one of the project's objectives was to work on pigment-producing fungi and bacteria.

6- He did not explain how to conduct biological evaluation experiments of natural pigments on rats: experimental design and number of rats

7- Acute toxicity tests (using 30 rats) and subacute toxicity tests (10 rats) were described, but the types of stains, concentrations, method of animal exposure, and types of organs used in the analysis were not explained.

8- He did not explain why he used female rats and not males

9- Calculating the sample size: It was not explained how the sample size was calculated.

10- The number of rats was mentioned as a total of 150, but in the experiments, 30 rats were mentioned in the acute toxicity experiments and 10 rats in the subacute toxicity experiments (clarification that must be mentioned).